Responses of Soil Microbial Communities in Soybean–Maize Rotation to Different Fertilization Treatments

Rotation and fertilization are important methods used to improve crop yield. In particular, rotation is an effective means of enhancing ecosystem diversity; however, there exist relatively few studies regarding the effects long-term maize–soybean on soil microbial communities. To further understand changes in community structure under fertilization, we a 9-year-old experimental site with as research object soybean continuous cropping control. We explored growth communities soybean–maize system treatments by analyzing physicochemical properties soil, agronomic traits, yield, structure. The results show that, comparison cropping, yield soybeans was increased 12.11% 21.42% different treatments, respectively. Additionally, significant increase nitrogen following combined fertilization. Moreover, pH, SOM, nutrient status were also improved. Bryobacter, Gemmatimonas, Rhodanobacter dominant bacteria. treatment relative abundance Bryobacter Rhodanobacter, Gemmatimonas. stability bacterial strengthened symbiotic relationship between species. prediction nitrogen-related functional genes indicates that ammonification nitrification. Heterocephalacria Mrakia fungal genera rotation. Saccharomyces significantly positively correlated ammonium levels Crop reduced Fusarium, but Fusarium. Functional gene plant pathogens reduced. This study provides theoretical basis for diversity service function